Laboratory OOI focused on biology research, means of prevention and therapy of pathogens of viral pathogens of emerging and socially significant diseases 3, 2 biohazard groups. Actively involved in countering the coronavirus pandemic. Conducts research on the persistence of coronavirus infectivity in the environment (Virus isolation article Cell and Organism Technologies for Assessment of the SARS-CoV-2 Infectivity in Fluid Environment A. A. Shelemba,¹ E. A. Kazachkova,¹ Yu. V. Kononova,¹ E. I. Kazachinskaya,¹ M. Yu. Rukavishnikov,² I. N. Kuvshinova,² M. I. Voevoda,¹ A. M. Shestopalov,¹ andA. A. Chepurnov¹   Bull Exp Biol Med. 2022; 173(4): 519–522.). Large-scale cultivation methods have been developed on various cell cultures in order to develop an inactivated whole-virion intranasal vaccine (Development of a Purified Viral Preparation for Studies of COVID-19 (SARS-CoV-2) Biology A. A. Chepurnov, E. I. Kazachinskaya, E. A. Kazachkova, K. A. Sharshov, Yu. V. Kononova, A. A. Shelemba, A. Yu. Alekseev, M. A. Gulyeva, M. I. Voevoda, andA. M. Shestopalov. Bull Exp Biol Med. 2021; 172(1): 49–52)

Techniques for the cultivation and titration of viruses by the methods of negative colonies and final dilutions were set..

Negative colony method, “plaques”, commonly used for counting the amount of virus in suspensions.

Cytopathic effect, caused in cell culture by many pathogens, including. SARS-Cov 2 also allows virus titration, or accumulate it to obtain purified concentrated preparations.

In the future, drugs are used for various studies., for example, to assess the immunomodulatory properties of individual viral proteins, obtained by electrophoretic separation.

And also to evaluate the sera of vaccinated and recovering patients by Western blot analysis or in an enzyme immunoassay.

The antiviral efficacy of natural compounds does not stand aside. Laboratory staff is investigating the anti-covid efficacy of a number of plant extracts (Kazachinskaya E.I., Chepurnov A. A., Kononova Yu.V., Romanyuk V.V., I.V. Romanyuk, Shestopalov A.M.. Obtaining an aqueous extract of fermented fireweed leaves (Ivan-tea), with inhibitory activity in vitro on SARS-COV-2 and herpes simplex virus 2 type. // Materials of the All-Russian scientific and practical conference "Safety and quality of medical care in infectious diseases. Sochi, 01-02 March 2022. S. 30-32. eLIBRARY ID: 48041821

Kazachinskaya E.I.,. Chepurnov A.A., Kononova Yu.V., Shelemba A.A., Romanyuk V.V., Magomedov M.G., Shestopalov A.M.. Inhibitory activity of tea compositions and their constituent ingredients on SARS-COV-2 replication in vitro.  // South of Russia: ecology, development. 2022. T. 17. N. 2. S. 76-90. DOI: 10.18470/1992-1098-2022-2-76-90.

Kazachinskaya E.I., Chepurnov A.A., Shelemba A.A., Huseynova S.A., Magomedov M.G., Kononova Yu.V., Romanyuk V.V., Shestopalov A.M.. Inhibitory activity of aqueous extracts of tea compositions, individual ingredients for their preparation and some plants for the replication of the herpes simplex virus 2 type in vitro. // South of Russia: ecology, development. 2022. T. 17. N3. S. 135-152. Doi.org/10.18470/1992-1098-2022-3-135-152

Kazachinskaya E.I., Romanova V.D., Ivanova A.V., ChepurnovA.A., Kononova Yu.V., Shaulo D.N., RomanyukV.V., Shestopalov A.M.. Inhibitory activity of dry ethanol extracts Artemisia spp. for SARS-CoV-2 replication in vitro. // South of Russia: ecology, development. 2022.T. 17. N 4. S. 111-129. DOI:  10.18470/1992-1098-2-22-4-111-129

Kazachinskaya E. I., Chepurnov A. A., Romanyuk V. IN., I. Romanyuk. IN., Kononova Yu. IN., Shestopalov A. M. “Method for obtaining an aqueous extract of leavesfireweed (Epilobium angustifoliumL.), exhibiting inhibitory activity against SARS-CoV-2 coronavirus and herpes simplex virus type 2in vitro”. //  Invention request 2022118966 (040062) Date priority11.07.2022. Received a notification of a positive result of a formal examination from 20.07.2022. At the request for examination on the merits of 04.10.2022 notification of the commencement of the substantive examination was received from 13.10.2022.)

Pseudovirus particle technologies enable biology studies of the most dangerous pathogens under normal laboratory conditions, which is actively used by laboratory staff (N-Heterocyclic borneol derivatives as inhibitors of Marburg virus glycoprotein-mediated VSIV pseudotype entry A A Kononova ¹, A S Sokolova ^1 2, S V Cheresiz ^1 3, About I Yarovaya ^1 2, R A Nikitina ^4 5, A A Chepurnov ^4 5, A G Pokrovsky ¹, N F Salakhutdinov ^1 2   Medchemcomm 2017 Oct 19;8(12):2233-2237.

Adapted Lethality: What We Can Learn from Guinea Pig-Adapted Ebola Virus Infection Model. S V Cheresiz ¹, E A Semenova ¹, A A Chepurnov ²  Adv Virol. 2016;2016:8059607.)

The modern instrumentation park makes it possible to assess the level of intermolecular interaction even at the stage of development of immunological preparations. Octet is an indispensable tool for studying receptor binding domains: Comparative Immunogenicity of the Recombinant Receptor-Binding Domain of Protein S SARS-CoV-2 Obtained in Prokaryotic and Mammalian Expression Systems Yuliia A Merkuleva etc. Vaccines (Basel). 2022 Jan 9;10(1):96. Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines? Yuliia A Merkuleva  etc. Viruses. 2022 May 16;14(5):1060.

New comfortable laboratory premises will contribute to the quality of research

Publications

Laboratory staff

1. Shelemba Arsenia Alexandrovna
   Senior Researcher, candidate of biological sciences
2. Kazachinskaya Elena Ivanovna
   Senior Researcher (inside. sovm.), Doctor of Biological Sciences
3. Romanova Valeria Dmitrievna
   laboratory assistant
4. Nikitina Raisa Alekseevna
   laboratory assistant