List of available methods/methods of the Center for Collective Use "Proteomic Analysis"

1. Conducting preparative isoelectric focusing of proteins by pI in a solution of small volumes (2,5 Jr).
2. Separation of proteins according to their isoelectric parameters in a gel for strips and glass tubes of various lengths and pH ranges.
3. Performing small and large vertical electrophoresis to separate proteins by mass, allowing you to simultaneously work with the number of gels up to 12 things, including in gels with density gradient distribution.
4. Immunoblot analysis using specific antibodies.
5. Staining gels and membranes using colorimetric, fluorescent, chemiluminescent dyes.
6. Color Registration, fluorescent and chemiluminescent images with subsequent processing and analysis of one- and two-dimensional gels and membranes, including for obtaining a two-dimensional "protein" map".
7. Cutting out protein gangs or dots from one- and two-dimensional gels and blots and preparation of samples for subsequent mass spectrometric analysis of proteins.
8. Electrophoretic separation of nucleic acids in agarose and acrylamide gels.
9. Chromato-mass-spectrometric analysis of peptides.
10. Targeted DNA sequencing.
11. Sanger sequencing.
12. Analysis of gene expression by polymerase chain reaction (PCR analysis).
13. Allele-specific PCR.
14. Real-time PCR analysis.
15. High performance liquid chromatography with spectrophotometric capability (UV/Vis), fluorescent and electrochemical detection.
16. Laser microdissection of preparations from frozen and fixed tissue samples using traditional microscopic methods: bright field, dark field, polarized light illumination, phase contrast and fluorescence.
17. Isolation with a laser microdissector of clones and cells of a certain type, followed by cultivation, study of cell interaction in culture.
18. Immunohistochemical analysis.
19. Evaluation of the toxicity of chemical compounds by double staining of cells with Hoechst and propidium iodide preparations, followed by calculation of the percentage of living dead and apoptotic cells.
20. Study of cell viability and proliferation.
21. Real-time cell detection: cell adhesion and migration analysis.
22. Acquisition and analysis of cell images in bright field.
23. Analysis of localization and distribution of fluorescently labeled proteins on fixed preparations of tissue sections.
24. Evaluation of the toxicity of mouse peritoneal macrophages by annexin and propidium iodide staining.
25. Analysis of localization and distribution of fluorescently labeled proteins in living cells.
26. Assessment of reactive oxygen species production in cells using DCF in real time.
27. Study of the penetration and excretion of fluorescent drugs into the cell in real time.
28. Assessment of phenotypic changes in cell populations by double staining of fixed cells with Hoechst and DiD preparations.
29. Live staining with fluorescent dyes to assess the morphology of intracellular organelles.
30. Analysis of the penetration of microparticles into cells by recording a fluorescent signal from microparticles.
31. Conducting drip digital PCR.