Areas of activity – screening of virus strains for the presence of oncolytic properties, study of the mechanisms of implementation of direct cytotoxic properties of oncolytic viruses and immunomodulatory antitumor effect on models of transplanted cell lines and on models of experimental oncogenesis in vivo; development of a form of a virus-containing substance with fixed physical and chemical parameters, established in the course of operating time optimization, purification and testing of anticancer properties.

Research methods - virological, serological, cell biology methods, morphological, immunohistochemical.

Tasks:

– search for new effective oncolytic strains and evaluation of antitumor activity on human tumor cell lines in vitro and animal models

– study of the molecular mechanisms of the direct cytotoxic antitumor effect of the studied virus strains and the mediated immunostimulatory effect depending on the method of virus administration, infectious dose, treatment regimens and other virotherapy parameters

– preparation of methodological solutions, related to the optimization of the production of strains, preparing it for dosage form, associated with the choice of storage conditions and the composition of the solutions used, environments and other necessary components

– obtaining a variant of the viral substance with high antitumor potential, safety for mammals and the stability of the genetic material during manipulations for the preparation of preparative material

Graduated in 2013 G. Novosibirsk State University (Faculty of Natural Sciences). AT 2019 year Yurchenko Ksenia Sergeevna defended her dissertation work on the topic “Study of the antitumor potential of wild strains of the Newcastle disease virus on human tumor cells and on the model of experimental oncogenesis in vivo” for the degree of Candidate of Biological Sciences.

The main direction of scientific research is the search and study of potential effective oncolytic infectious agents, predominantly among the Paramyxovirus family, on the basis of which it is possible to create a prototype anticancer drug for virotherapy of tumors in humans and mammals.

Yurchenko K.S.. primary screening of oncolytic properties was carried out on a panel of transplantable human tumor cell lines and Newcastle disease virus strains were selected, with potential anticancer properties. Strains with the most pronounced cytolytic effect in vitro were studied in virotherapy of experimental tumors. – intratumoral virotherapy of xenograft of human non-small cell lung carcinoma, syngeneic murine Krebs-2 carcinoma and intravenous virotherapy for Lewis lung carcinoma and melanoma B6. When studying the antitumor properties of Newcastle disease virus strains, it was found, that virotherapy contributes to the inhibition of tumor progression of solid tumor nodes. An increase in destructive changes in the treated tumor tissue and a significant decrease in the number of vessels in the tumor node over the course of the study were shown in comparison with untreated tumor-carrying animals., which indicates the ability of the virus to directly or indirectly affect the neoangiogenesis of a developing tumor, regulating tissue trophism. Possible mechanisms of virus-mediated activation of tumor cell death were studied.. Installed, that in virus-infected transplanted human tumor cells, the mechanism for implementing the antitumor properties of strains with different virolence proceeds to a greater extent along the apoptosis pathway and leads to the rapid implementation of the apoptotic program. However,, that necroptosis competes with the apoptotic program, as shown in experimental work with inhibition of the main effectors of cell death pathways.

Publications

Laboratory staff

1. Gulyaeva Marina Alexandrovna
   Researcher, candidate of biological sciences
2. Lyubov Sergeevna Adamenko
   junior researcher
3. Alexandra Vladimirovna Hlushchenko
   junior researcher
4. Karkavin Yury Igorevich
   junior researcher
5. Andreeva Elizaveta Olegovna
   laboratory assistant
6. Skorupo Anna Sergeevna
   laboratory assistant
7. Fedotova Marina Sergeevna
   laboratory assistant
8. Filippova Anastasia Yurievna
   laboratory assistant